Device

Part:BBa_K2230010:Design

Designed by: Yi-Lun Huang   Group: iGEM17_Mingdao   (2017-10-19)


Pcar-RBS-PhlF-T/pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

Pcar-RBS-PhlF was amplified from RBS + PhlF repressor + terminator (BBa_K1725041) using a primer with Pcar-RBS (B0032) sequence. The PCR product was ligated to pSB1C3 cut by XbaI and PstI.


References